Evaluation of real-time reverse transcriptase PCR and real-time loop-mediated amplification assays for severe acute respiratory syndrome coronavirus detection.
Identifieur interne : 004840 ( Main/Exploration ); précédent : 004839; suivant : 004841Evaluation of real-time reverse transcriptase PCR and real-time loop-mediated amplification assays for severe acute respiratory syndrome coronavirus detection.
Auteurs : Leo L M. Poon [Hong Kong] ; Bonnie W Y. Wong ; Kwok H. Chan ; Stella S F. Ng ; Kwok Y. Yuen ; Yi Guan ; J S Malik PeirisSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 2005.
Descripteurs français
- KwdFr :
- Humains, Partie nasale du pharynx (virologie), RT-PCR (), Reproductibilité des résultats, Sensibilité et spécificité, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (virologie), Techniques d'amplification d'acides nucléiques (), Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Partie nasale du pharynx, Syndrome respiratoire aigu sévère.
- Humains, RT-PCR, Reproductibilité des résultats, Sensibilité et spécificité, Techniques d'amplification d'acides nucléiques.
English descriptors
- KwdEn :
- Humans, Nasopharynx (virology), Nucleic Acid Amplification Techniques (methods), Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction (methods), SARS Virus (genetics), SARS Virus (isolation & purification), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (virology).
- MESH :
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- methods : Nucleic Acid Amplification Techniques, Reverse Transcriptase Polymerase Chain Reaction.
- virology : Nasopharynx, Severe Acute Respiratory Syndrome.
- Humans, Reproducibility of Results, Sensitivity and Specificity.
Abstract
We compared the performance of a recently established real-time loop-mediated amplification (LAMP) assay with the one from a highly sensitive quantitative PCR assay. None of these assays produced false-positive results in this study. For samples isolated from patients within the first 3 days of disease onset, the detection rate of the quantitative PCR assay was higher (14 of 15 were positive) than the LAMP assay (9 of 15 were positive). By contrast, the detection rates of these assays toward specimens sampled from patients with more than 3 days of illness were similar (32 of 44 for PCR and 33 of 44 for LAMP were positive). The simpler operation of LAMP might be a possible solution for on-site diagnosis.
DOI: 10.1128/JCM.43.7.3457-3459.2005
PubMed: 16000477
Affiliations:
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Le document en format XML
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Poon</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR. llmpoon@hkucc.hku.hk</nlm:affiliation><country wicri:rule="url">Hong Kong</country></affiliation></author><author><name sortKey="Wong, Bonnie W Y" sort="Wong, Bonnie W Y" uniqKey="Wong B" first="Bonnie W Y" last="Wong">Bonnie W Y. Wong</name></author><author><name sortKey="Chan, Kwok H" sort="Chan, Kwok H" uniqKey="Chan K" first="Kwok H" last="Chan">Kwok H. Chan</name></author><author><name sortKey="Ng, Stella S F" sort="Ng, Stella S F" uniqKey="Ng S" first="Stella S F" last="Ng">Stella S F. Ng</name></author><author><name sortKey="Yuen, Kwok Y" sort="Yuen, Kwok Y" uniqKey="Yuen K" first="Kwok Y" last="Yuen">Kwok Y. Yuen</name></author><author><name sortKey="Guan, Yi" sort="Guan, Yi" uniqKey="Guan Y" first="Yi" last="Guan">Yi Guan</name></author><author><name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J S Malik" last="Peiris">J S Malik Peiris</name></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="ISSN">0095-1137</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Humans</term><term>Nasopharynx (virology)</term><term>Nucleic Acid Amplification Techniques (methods)</term><term>Reproducibility of Results</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Sensitivity and Specificity</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Humains</term><term>Partie nasale du pharynx (virologie)</term><term>RT-PCR ()</term><term>Reproductibilité des résultats</term><term>Sensibilité et spécificité</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Techniques d'amplification d'acides nucléiques ()</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Nucleic Acid Amplification Techniques</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Partie nasale du pharynx</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Nasopharynx</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>RT-PCR</term><term>Reproductibilité des résultats</term><term>Sensibilité et spécificité</term><term>Techniques d'amplification d'acides nucléiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We compared the performance of a recently established real-time loop-mediated amplification (LAMP) assay with the one from a highly sensitive quantitative PCR assay. None of these assays produced false-positive results in this study. For samples isolated from patients within the first 3 days of disease onset, the detection rate of the quantitative PCR assay was higher (14 of 15 were positive) than the LAMP assay (9 of 15 were positive). By contrast, the detection rates of these assays toward specimens sampled from patients with more than 3 days of illness were similar (32 of 44 for PCR and 33 of 44 for LAMP were positive). The simpler operation of LAMP might be a possible solution for on-site diagnosis.</div></front></TEI><affiliations><list><country><li>Hong Kong</li></country></list><tree><noCountry><name sortKey="Chan, Kwok H" sort="Chan, Kwok H" uniqKey="Chan K" first="Kwok H" last="Chan">Kwok H. Chan</name><name sortKey="Guan, Yi" sort="Guan, Yi" uniqKey="Guan Y" first="Yi" last="Guan">Yi Guan</name><name sortKey="Ng, Stella S F" sort="Ng, Stella S F" uniqKey="Ng S" first="Stella S F" last="Ng">Stella S F. Ng</name><name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J S Malik" last="Peiris">J S Malik Peiris</name><name sortKey="Wong, Bonnie W Y" sort="Wong, Bonnie W Y" uniqKey="Wong B" first="Bonnie W Y" last="Wong">Bonnie W Y. Wong</name><name sortKey="Yuen, Kwok Y" sort="Yuen, Kwok Y" uniqKey="Yuen K" first="Kwok Y" last="Yuen">Kwok Y. Yuen</name></noCountry><country name="Hong Kong"><noRegion><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L M" last="Poon">Leo L M. Poon</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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